![]() Nishikawa,Ĭreative Commons Attribution-NonCommercial 3.0 Unported Licence. These results indicate that DNA nanostructures with high DNA density are suitable for delivery to immune cells.ĭNA density-dependent uptake of DNA origami-based two-or three-dimensional nanostructures by immune cells There was a positive correlation between the density and cellular uptake. Flow cytometry analysis revealed that the uptake of DNA nanostructures by murine macrophage-like RAW264.7 cells was higher for those with higher DNA density than those with low density. Agarose gel electrophoresis and atomic force microscopy showed that all of the nanostructures were prepared with high yield. ![]() The DNA density estimated from the average distance between any two of the ten DNA helices in the DNA nanostructures was different among them Rec50, Rec0 and Rec50/50 had a higher density than Rec180 and Rec90. The fluctuation, or flexibility, of these DNA nanostructures under solution conditions was estimated using CanDo software. Rec50/50 has two bends of 50 degrees each so that the both ends stick together to form a triangular prism shape. Rec90, Rec50 and Rec0 were bent forms of Rec180 at the center by 90, 50 or 0 degrees, respectively. Rec180 had a rectangular-shaped, almost flat structure. We compared the following five types of DNA nanostructures, all of which consisted of ten DNA helices using an identical circular, single-stranded scaffold and staples. To examine this, DNA nanostructures with almost identical molecular weights and structural flexibility, but with different shapes and DNA densities, were designed using DNA origami technology. In this study, we focused on the DNA density that can be important for the their recognition and uptake by immune cells. However, the structural properties of DNA nanostructures required for the delivery have not fully been elucidated. ![]() DNA nanostructures are expected to be applied for targeted drug delivery to immune cells.
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